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KMID : 0545120100200061011
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 6 p.1011 ~ p.1017
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New Dioscin-glycosidase Hydrolyzing Multi-glycosides of Dioscin from Absidia Strain
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Fu Yao-Yao
Yu Hong-Shan
Tang Si-Hui
Hu Xiang-Chun
Wang Yuan-Hao
Liu Bing
Yu Chen-Xu
Jin Feng-Xie
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Abstract
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A novel dioscin-glycosidase that specifically hydrolyzes multi-glycosides such as 3-O-α-L-(1→4)-rhamnoside, 3-O-α-L-(1→2)-rhamnoside, 3-O-α-L-(1→4)-arabinoside and β-D-glucoside on diosgenin was isolated from Absidia sp.d38 strain; and it was purified and characterized. The molecular weight of the new dioscin-glycosidase is about 55 kDa in SDS-PAGE. The the dioscin-glycosidase gradually hydrolyzes either 3-O-α-L-(1→4)-Rha or 3-O-α-L-(1→2)-Rha of dioscin to 3-O-α-L-Rha-β-D-Glc-diosgenin; further rapidly hydrolyzes the other α-L-Rha of 3-O-α-L-Rha-β-D-Glc-diosgenin to main intermediate products 3-O-β-D-glc-diosgenin; and subsequently hydrolyzes intermediate products to final product of aglycone; the new enzyme also gradually hydrolyzes 3-O-α-L-(1→4)-arabinoside, 3-O-α-L-(1→2)-rhamnoside and β-D-glucoside of [3-O-α-L-(1→4)-Ara, 3-O-α-L-(1→2)-Rha]-β-D-Glc-diosgenin into final product diosgenin, exhibiting significant differences from previously reported glycosidases. The optimal temperature of the new dioscin-glycosidase is 40?C; the optimal pH is 5.0. The activity of the new dioscin-glycosidase was not affected by the Na+, K+ and Mg2+ ions; it was significantly inhibited by the Cu2+ and Hg2+ ions; and it was slightly affected by the Ca2+ ions.
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KEYWORD
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Dioscin, Dioscin-glycosidase, Hydrolyzing multi-glycosides
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